Journal: Heliyon

Article Title: Advanced cell-based products generated via automated and manual manufacturing platforms under the quality by design principle: Are they equivalent or different?

doi: 10.1016/j.heliyon.2023.e15946

Figure Lengend Snippet: Immunomodulatory effects and metabolic activities of UC-MSCs manufactured with the Quantum Cell Expansion system and HYPERflasks. (A) Proliferation of CD4 + T cells in the presence of P4-MSCs, QUANT-MSCs, and HYPER/MSCs. Percentage of CD4 + T cell proliferation in the presence of P4-MSCs, HYPER-MSCs, and QUANT-MSCs at PBMC:MSC ratios of 10:1 and 20:1 compared to the control (PBMCs only). (B) Proliferation of CD8 + T cells in the presence of P4-MSCs, QUANT-MSCs, and HYPER/MSCs. Percentage of CD8 + T cell proliferation under the same conditions as in (A). Results demonstrate that both QUANT-MSCs and HYPER-MSCs significantly suppress the proliferation of CD4 + (A) and CD8 + (B) T cells. No significant differences were observed in the suppression of T cell proliferation among P4-MSCs, HYPER-MSCs, and QUANT-MSCs, indicating that both HYPERFlask and Quantum manufacturing methods did not alter the immunoregulatory effects of hUC-MSCs. (C) Glycolysis capacity of P4-MSCs, QUANT-MSCs, and HYPER/MSCs. QUANT-MSCs show reduced glycolysis and glycolytic capacity compared to P4-MSCs and HYPER-MSCs, with no significant differences in basal glycolytic rate or glycolytic reserve among the groups. (D) Mitochondrial respiratory capacity of P4-MSCs, QUANT-MSCs, and HYPER/MSCs including basal respiration rate, proton leakage, maximal respiration, and ATP production, are similar among P4-MSCs, QUANT-MSCs, and HYPER-MSCs. QUANT-MSCs exhibit a higher spare respiratory capacity than input cells (P4-MSCs) and HYPER-MSCs.

Article Snippet: The cells were counted and seeded into Corning® CellBIND® Surface HYPERFlask® cell culture vessels at a density of 3400 cells/cm 2 .

Techniques: Control